HSP70 Antibody (Acetyl Lys77): PerCP

SKU: orb414158

Description

Rabbit polyclonal antibody against HSP70 Acetyl Lys77 conjugated to PerCP

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases,Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Cancer | Tumor Biomarkers

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution Range: WB (1:1000); ELISA (1:1000); ICC/IF (1:60); IHC (1:50)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution of SPC-743 was sufficient for detection of HSP70 Acetyl Lys77 in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the N-terminal of Human HSP70 Acetyl Lys77 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Target: HSP70 Acetyl Lys77
• Molecular Weight: 70 kDa
• Conjugation: PerCP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 0.5mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSP70 Acetylated lysine 77 antibody, HSP70 acLys77 antibody, HSP70 acK77 antibody, Acetylated Lysine HSP70 antibody, HSP70 AcetylLys77 antibody, HSP70 AceyltK77 antibody, DnaK type molecular chaperone HSP70 1 antibody, Epididymis secretory protein Li 103 antibody, Heat shock 70 kDa protein 1 antibody, Heat shock 70 kDa protein 1/2 antibody, Heat shock 70 kDa protein 1A/1B antibody, Heat shock 70kDa protein 1A antibody, Heat shock 70kDa protein 1B antibody, Heat shock induced protein antibody, HEL S 103 antibody, HSP70 1 antibody, HSP70 1B antibody, HSP70 2 antibody, HSP70-1/HSP70-2 antibody, HSP70-1A antibody, HSP70.1 antibody, HSP70.1/HSP70.2 antibody, HSP70I antibody, HSP71_HUMAN antibody, HSP72 antibody, HSPA1 antibody, HSPA1A antibody, HSPA1B antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 μM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite.

Immunohistochemistry analysis using Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody. Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70 Acetyl Lys77 protein using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.

Quick Database Links

Gene Symbol

HSP70 Acetyl Lys77

UniProt

P0DMV9, P0DMV8

NCBI Gene ID

3303

RefSeq (Protein):NP_005336.3