HSP70 Antibody: HRP

SKU: orb151243

Description

Rabbit polyclonal to Hsp70 (HRP). Hsp70 genes encode abundant heat-inducible 70-kDa hsps Hsp70 genes encode abundant heat-inducible 70-kDa hsps...

Research Area

Images & Validation

−

Item 1 of 7

Tested Applications	ELISA, IHC, WB
Dilution range	WB (1:1000), IHC (1:100), ICC/IF (1:100), IP (1:100)
Reactivity	Bovine, Canine, Fish, Guinea pig, Hamster, Human, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rat, Sheep
Application Notes	A 1:1000 dilution of SPC-103 was sufficient for detection of HSP70 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Full length HSP70 Protein
Target	HSP70
Molecular Weight	70kDa
Conjugation	HRP

Storage & Handling

−

Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

−

HSP70 1 antibody, HSP70 2 antibody, HSP70.1 antibody, HSP72 antibody, HSP73 antibody, HSPA1 antibody, HSPA1A antibody, HSPA1B antibody

HSP70/HSC70 Antibody: HRP [orb146788]

Applications:	ELISA, IHC, WB
Reactivity:	Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Rabbit, Rat, Sheep, Yeast
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	HRP

Sizes Available: 200 μg

Item 1 of 1
Zebrafish HeatShock Protein 70 (HSP-70/HSPA9) ELISA Kit [orb1088148]
Reactivity:
Zebrafish
Dynamic Range:
3.13-200 ng/mL
Sensitivity:
1.33 ng/mL
Sizes Available: 96 T, 48 T
Item 1 of 1
Horse HeatShock Protein 70 (HSP-70/HSPA9) ELISA Kit [orb1146943]
Reactivity:
Equine
Dynamic Range:
1.57-100 ng/mL
Sensitivity:
0.53 ng/mL
Sizes Available: 48 T, 96 T
Item 1 of 1
Goat HeatShock Protein 70 (HSP-70/HSPA9) ELISA Kit [orb1146821]
Reactivity:
Goat
Dynamic Range:
3.13-200 ng/mL
Sensitivity:
1.35 ng/mL
Sizes Available: 96 T, 48 T

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp70 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody at 1:50000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.

Western blot analysis of Human, Rat brain cell lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody. Load: 2 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody at 1:10000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Western blot analysis of Mouse Pam212 cells showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp70 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody. Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody at 1:1000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.

Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.

Quick Database Links

Gene Symbol

HSP70

UniProt

P0DMV9, P0DMV8

NCBI Gene ID

3303

RefSeq (Protein):NP_005336.3

UniProt Details

−

No UniProt data available

NCBI Gene Details

−

No NCBI Gene data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005336.3

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols