hsp90a.1 Antibody (Center)

SKU: orb420761

Description

Rabbit polyclonal antibody to hsp90a.1

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Crown Antibodies;Primary Antibodies;Antibody Collections;Signal Transduction;Zebrafish;Catalog Updated

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:2000, IHC-P: 1:25
• Reactivity: Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB47443
• Immunogen: Synthetic Peptide
• Target: hsp90a.1
• Molecular Weight: 83319
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heat shock protein HSP 90-alpha 1, hsp90a1, hsp90, hsp90a, hsp90aa1

Western blot analysis of lysate from zebra fish heart tissue lysate, using (DANRE) hsp90a. 1 Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

Immunohistochemical analysis of paraffin-embedded Z. skeletal muscle section using hsp90a. 1 Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution + ZF4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution. Lane 1: Zebrafish muscle lysate. Lane 2: Zebrafish lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution. Lane 1: Zebrafish muscle lysate. Lane 2: Zebrafish lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

hsp90a.1

UniProt

Q90474