HSPA1B monoclonal antibody (M02), clone 3B7

SKU: orb2292767

Description

Mouse monoclonal antibody raised against a partial recombinant HSPA1B.

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, PLA, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone No.: 3B7
• Immunogen: HSPA1B (NP_005336, 531 a.a. ~ 611 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: VQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLY
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection limit for recombinant GST tagged HSPA1B is approximately 0.03 ng/ml as a capture antibody.

HSPA1B monoclonal antibody (M02), clone 3B7 Western Blot analysis of HSPA1B expression in IMR-32.

HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in NIH/3T3.

HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in PC-12.

HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in Raw 264.7.

Immunofluorescence of monoclonal antibody to HSPA1B on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml]

Proximity Ligation Analysis of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-HSPA1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot detection against Immunogen (34.65 KDa).

Quick Database Links

RefSeq (Protein):NP_005336