HSPB1 Antibody, KO Validated

SKU: orb1274486

Description

HSPB1 Antibody, KO Validated

Research Area

,Apoptosis,Cancer,Cell Cycle,Signal Transduction,Stem Cell

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1).
• Target: HSPB1
• Molecular Weight: Observed: 25kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27, HEL-S-102

Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody (orb1274486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibody (orb1274486) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using HSPB1 antibody (orb1274486) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

HSPB1

UniProt

P04792