HSPB1 Antibody [SAIC-23C-35], Rabbit IgG

SKU: orb1672065

Description

HSPB1 Antibody [SAIC-23C-35], Rabbit IgG

Research Area

,Immunology

Images & Validation

• Tested Applications: WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Recombinant
• Isotype: IgG kappa
• Clone No.: SAIC-23C-35
• Immunogen: Peptide "VSLDVNHFAPDELTVK" derived from Heat shock protein beta-1, involved in stress resistance and actin organisation, conjugated to KLH.
• Target: BCL5
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heat shock protein beta-1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSP27, HSP28

Western Blot using anti-HSPB1 antibody SAIC-23C-35. HeLa(A) (0.03 ug/ml)- MCF7(B) (0.001 ug/ml)- Caco-2(C) (0.1 ug/ml) and A431(D) (0.03 ug/ml) cell lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-23C-35 (orb1672065)- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry using the Anti-HSPB1 antibody SAIC-23C-35. Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-23C-35 (orb1672065 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-HSPB1 (SAIC-23C-35). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-23C-35 (orb1672065) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672065- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

BCL5

UniProt

P04792