HSPD1 Antibody (Center)

SKU: orb1788345

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Viral process;Host-virus interaction;Immune system process;Mitochondrion;Stress response

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:10~50, IF: 1:100, WB: 1:1000
• Reactivity: Human, Mouse
• Predicted Reactivity: Hamster, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: 187-215 aa
• Target: This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-215 amino acids from the Central region of human HSPD1.
• Molecular Weight: 61055 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPD1; HSP60; 60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; Chaperonin 60; Heat shock protein 60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein

Western blot analysis of lysates from A431, mouse NIH/3T3, rat PC-12 cell line (from left to right), using HSPD1 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Fluorescent image of U251 cells stained with HSPD1 (Center) antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with HSPD1 (Center) primary antibody (1: 100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min).

Fluorescent confocal image of Hela cell stained with HSPD1 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HSPD1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HSPD1 immunoreactivity is localized to Mitochondria significantly.

Quick Database Links

Gene Symbol

This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-215 amino acids from the Central region of human HSPD1.

UniProt

P10809

RefSeq (Protein):NP_002147.2, NP_955472.1