HSPD1 Antibody

SKU: orb1264552

Description

HSPD1 Antibody

Research Area

,Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Gallus, Hamster, Mouse, Rat
• Application Notes:
  For WB starting dilution is: 1:1000For IF starting dilution is: 1:100

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-215 amino acids from the Central region of human HSPD1.
• Target: HSPD1
• Molecular Weight: 61 kDa
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60

Western blot analysis in A431 cell line lysates (35 ug/lane).

Fluorescent image of U251 cells stained with HSPD1 antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with HSPD1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Note the highly specific localization of the HSPD1 mainly to the mitochondria, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000144381).

Fluorescent confocal image of Hela cell stained with HSPD1 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HSPD1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HSPD1 immunoreactivity is localized to Mitochondria significantly.

Quick Database Links

Gene Symbol

HSPD1

UniProt

P10809

RefSeq:P10809