Human 4-Hydroxynonenal ELISA Kit

SKU: orb782167

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HNE protein.

Research Area

Product Categories/ELISA Kits,Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  Standard: 2500 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve

  Read more...

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates
• Range: 39.07-2500 pg/mL
• Sensitivity: 19.1 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal ELISA Kit