Human ATG5 protein

SKU: orb391270

Description

Recombinant human ATG5 protein is produced by our E. coli expression system and the target gene encoding Met1-Asp275 is expressed.

Research Area

Product Categories/Products/Proteins,Infectious Diseases,Signaling Pathways/Autophagic,Immunology,Product Categories/Research Area,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Product Categories/Proteins,Cancer

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: ATG5
• Reactivity: Human
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI TVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLK EVCPSAIDPE DGEKKNQVMI HGIEPMLETP LQWLSEHLSY PDNFLHISII PQPTD

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 20mM Tris, 0.2M NaCl, 1mM DTT, 40% Glycerol, pH 8.0.
• Buffer/Preservatives: Liquid in a 0.2 μM filtered solution of 20mM Tris, 0.2M NaCl, 1mM DTT, 40% Glycerol, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein

Quick Database Links

Gene Symbol

ATG5

UniProt

Q9H1Y0

NCBI Gene ID

9474