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Human BIRC5 protein

SKU: orb391297

Description

Recombinant human BIRC5 protein is produced by our E. coli expression system and the target gene encoding Met1-Asp142 is expressed.

Research Area

Product Categories/Products/Proteins,Product Categories/Research Area,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Product Categories/Proteins,Cancer

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetBIRC5
ReactivityHuman
EndotoxinsEndotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceMGAPTLPPAW QPFLKDHRIS TFKNWPFLEG CACTPERMAE AGFIHCPTEN EPDLAQCFFC FKELEGWEPD DDPIEEHKKH SSGCAFLSVK KQFEELTLGE FLKLDRERAK NKIAKETNNK KKEFEETAKK VRRAIEQLAA MD

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM Tris, 0.1M NaCl, pH 7.5.
Buffer/PreservativesLyophilized from a 0.2 μM filtered solution of 20mM Tris, 0.1M NaCl, pH 7.5.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 250.00
50 μg
$ 600.00
500 μg
$ 3,860.00
DispatchUsually dispatched within 5-10 working days
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