Human BLK protein

SKU: orb391298

Description

Recombinant human BLK protein is produced by our E. coli expression system and the target gene encoding Gly2-Pro505 is expressed with a 6His tag at the C-terminus.

Research Area

Product Categories/Products/Proteins,Product Categories/Research Area,Product Categories/Proteins,Epigenetics

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: BLK
• Reactivity: Human
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: GLVSSKKPDK EKPIKEKDKG QWSPLKVSAQ DKDAPPLPPL VVFNHLTPPP PDEHLDEDKH FVVALYDYTA MNDRDLQMLK GEKLQVLKGT GDWWLARSLV TGREGYVPSN FVARVESLEM ERWFFRSQGR KEAERQLLAP INKAGSFLIR ESETNKGAFS LSVKDVTTQG ELIKHYKIRC LDEGGYYISP RITFPSLQAL VQHYSKKGDG LCQRLTLPCV RPAPQNPWAQ DEWEIPRQSL RLVRKLGSGQ FGEVWMGYYK NNMKVAIKTL KEGTMSPEAF LGEANVMKAL QHERLVRLYA VVTKEPIYIV TEYMARGCLL DFLKTDEGSR LSLPRLIDMS AQIAEGMAYI ERMNSIHRDL RAANILVSEA LCCKIADFGL ARIIDSEYTA QEGAKFPIKW TAPEAIHFGV FTIKADVWSF GVLLMEVVTY GRVPYPGMSN PEVIRNLERG YRMPRPDTCP PELYRGVIAE CWRSRPEERP TFEFLQSVLE DFYTATERQY ELQPLEHHHH HH

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 20mM Tris, 500mM NaCl, 1mM DTT, pH 7.4.
• Buffer/Preservatives: Liquid in a 0.2 μM filtered solution of 20mM Tris, 500mM NaCl, 1mM DTT, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tyrosine-protein kinase Blk; B lymphocyte kinase; p55-Blk

Quick Database Links

Gene Symbol

BLK

UniProt

P51451

NCBI Gene ID

640