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Human CASP10 protein

SKU: orb391355

Description

Recombinant human CASP10 protein is produced by our E. coli expression system and the target gene encoding Val220-Ile480 is expressed with a 6His tag at the C-terminus.

Research Area

Product Categories/Products/Proteins,Product Categories/Research Area,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Product Categories/Proteins,Cancer

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetCASP10
ReactivityHuman
EndotoxinsEndotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceMVKTFLEALP QESWQNKHAG SNGNRATNGA PSLVSRGMQG ASANTLNSET STKRAAVYRM NRNHRGLCVI VNNHSFTSLK DRQGTHKDAE ILSHVFQWLG FTVHIHNNVT KVEMEMVLQK QKCNPAHADG DCFVFCILTH GRFGAVYSSD EALIPIREIM SHFTALQCPR LAEKPKLFFI QACQGEEIQP SVSIEADALN PEQAPTSLQD SIPAEADFLL GLATVPGYVS FRHVEEGSWY IQSLCNHLKK LVPRHEDILS ILEHHHHHH

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 25mM HEPES, 10mM DTT, pH 7.5.
Buffer/PreservativesLiquid in a 0.2 μM filtered solution of 25mM HEPES, 10mM DTT, pH 7.5.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ALPS2; apoptotic protease Mch-4; CASP-10; CASPA; caspase-10; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-convert

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Datasheet
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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human CASP10 protein (orb391355)

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Available Sizes

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10 μg
$ 340.00
50 μg
$ 910.00
500 μg
$ 3,390.00
DispatchUsually dispatched within 5-10 working days
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