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Human CD274 protein

SKU: orb706872

Description

Recombinant Human CD274 Protein is produced by mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a mFc tag at the C-terminus.

Research Area

Product Categories/Products/Proteins,Immunology,Product Categories/Research Area,Epigenetics,Product Categories/Proteins

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
TargetCD274
ReactivityHuman
EndotoxinsEndotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceFTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE NHTAELVIPE LPLAHPPNER TPRDCGCKPC ICTVPEVSSV FIFPPKPKDV LTITLTPKVT CVVVDISKDD PEVQFSWFVD DVEVHTAQTQ PREEQFNSTF RSVSELPIMH QDWLNGKEFK CRVNSAAFPA PIEKTISKTK GRPKAPQVYT IPPPKEQMAK DKVSLTCMIT DFFPEDITVE WQWNGQPAEN YKNTQPIMDT DGSYFVYSKL NVQKSNWEAG NTFTCSVLHE GLHNHHTEKS LSHSPGK

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Buffer/PreservativesLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

B7H1 protein, PDCD1L1 protein, PDCD1LG1 protein, PDL1 protein, Programmed cell death 1 ligand 1 protein, PD-L1 protein, PDCD1 ligand 1 protein, Programmed death ligand 1 protein, B7 homolog 1 protein, B7-H1 protein, CD274 protein

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 200.00
50 μg
$ 490.00
500 μg
$ 2,410.00
DispatchUsually dispatched within 5-10 working days
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