Human CD3 epsilon Protein, His Tag

SKU: orb257326

Description

Human CD3 epsilon, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).

Research Area

Disease Biomarkers

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. Maybe caused by unknown PTMs, the protein migrates as 18-19 kDa and 21-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition, and 35-43 kDa under non-reducing (NR) condition (SDS-PAGE).

Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Target: CD3 epsilon
• Reactivity: Human
• Tag: C-10×His
• Expression Region: Asp 23 - Asp 126
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 16.9 kDa
• Purity: 90%
• Protein Sequence: NP_000724.1

Storage & Handling

• Storage: -20°C to -70°C for 24 months in lyophilized state;. -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of Human CD3 epsilon protein

Immobilized Anti Human CD3 MAb, mouse IgG1 at 2 ug/mL (100 uL/well) can bind Human CD3E, His Tag with a linear range of 0.4-6.25 ng/mL.

Quick Database Links

Gene Symbol

CD3 epsilon

UniProt

P07766

RefSeq (Protein):NP_000724.1