Human CD3E & CD3G Heterodimer Protein, Fc-His Tag & Fc-Flag Tag

SKU: orb348813

Description

Human CD3E & CD3G Heterodimer Protein, Fc, His Tag & Fc, Flag Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)).

Research Area

Disease Biomarkers

Images & Validation

• Application Notes:
  Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.

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Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Target: CD3E & CD3G
• Reactivity: Human
• Tag: C-Fc & C-6×His | C-Fc & C-Flag
• Expression Region: Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 39.2 kDa (CD3E) & 38.6 kDa (CD3G)
• Purity: 95%
• Protein Sequence: NP_000724.1 (CD3E) & AAI13831.1 (CD3G)

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

CD3E & CD3G

UniProt

P07766, P09693

RefSeq (Protein):NP_000724.1

RefSeq:AAI13831.1, CD3