Human Collagen IX protein

SKU: orb391504

Description

Recombinant human Collagen IX protein is produced by our mammalian expression system and the target gene encoding Ala24-Pro328 is expressed with a 6His tag at the C-terminus.

Research Area

Product Categories/Products/Proteins,Product Categories/Research Area,Product Categories/Proteins,Epigenetics

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: COL9A1
• Reactivity: Human
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: AVKRRPRFPV NSNSNGGNEL CPKIRIGQDD LPGFDLISQF QVDKAASRRA IQRVVGSATL QVAYKLGNNV DFRIPTRNLY PSGLPEEYSF LTTFRMTGST LKKNWNIWQI QDSSGKEQVG IKINGQTQSV VFSYKGLDGS LQTAAFSNLS SLFDSQWHKI MIGVERSSAT LFVDCNRIES LPIKPRGPID IDGFAVLGKL ADNPQVSVPF ELQWMLIHCD PLRPRRETCH ELPARITPSQ TTDERGPPGE QGPPGPPGPP GVPGIDGIDG DRGPKGPPGP PGPAGEPGKP GAPGKPGTPG ADTSPVDHHH HHH

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Buffer/Preservatives: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Collagen alpha-1(IX) chain

Quick Database Links

Gene Symbol

COL9A1

UniProt

P20849

NCBI Gene ID

1297