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Description
Research Area
Product Categories/Products/Proteins,Product Categories/Research Area,Epigenetics,PSC Type: Protein
Images & Validation
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Item 1 of 1
| Tested Applications | ELISA, IHC, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human |
|---|---|
| Biological Origin | Human |
| Target | COL2A1 |
| Conjugation | Unconjugated |
| Purity | Human Collagen Type II has been prepared from adult human knee cartilage and is chromatographically and immunologically pure. Human Collagen Type II is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Human Collagen Type II reacts with anti-Collagen Type II. Reaction with Rockland’s anti-Collagen I, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA). |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide. 0.5 M Acetic Acid |
| Concentration | 1.0 mg/mL |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Type II collagen protein, collagen II protein, human collagen protein, hyaline collagen protein
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> 90%, determined by SDS-PAGE
This protein contains the amino acids (Asp 1242-Leu 1487) of human COL2A1 isoform 1 (P02458-2), known as chondrocalcin, was expressed with a polyhistidine tag at the N-terminus and expressed from HEK293 Cells.
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> 92%, determined by SDS-PAGE
This protein contains the amino acids (Asp 1242-Leu 1487) of human COL2A1 isoform 1 (P02458-2), known as chondrocalcin, was expressed with a polyhistidine tag at the N-terminus and expressed from HEK293 Cells.
50 μg, 20 μg
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | CAA34683.1 |
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
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