Human Complement C5a Protein

SKU: orb257232

Description

Human Complement C5a, Tag Free is expressed from E. coli cells. It contains AA Leu 679 - Arg 751 (Accession # P01031).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries no "tag". The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Key Properties

• Expression System: E. coli
• Biological Origin: Human
• Biological Activity: Immobilized Human Complement C5a, Tag Free at 2 µg/mL (100 µL/well) can bind Anti-C5a MAb, Human IgG1 with a linear range of 0.8-13 ng/mL (QC tested).
• Target: Complement C5a
• Reactivity: Human
• Tag: Native
• Expression Region: Leu 679 - Arg 751
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 8.3 kDa
• Purity: 95%
• Protein Sequence: Leu 679 - Arg 751

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of Human C5a protein

Immobilized Human Complement C5a, Tag Free (Cat. No. orb257232) at 2 μg/mL (100 μL/well) can bind Anti-C5a MAb, Human IgG1 with a linear range of 0.8-13 ng/mL (QC tested).

Quick Database Links

Gene Symbol

Complement C5a

UniProt

P01031