Human CX3CL1 protein

SKU: orb82612

Description

Fractalkine, also named neurotactin, is a novel chemokine recently identified through bioinformatics. Fractalkine has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, fractalkine is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human fractalkine cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human fractalkine can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble fractalkine. The soluble chemokine domain of human fractalkine was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse fractalkine was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.

Images & Validation

• Tested Applications: HPLC, SDS-PAGE
• Application Notes:
  Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Key Properties

• Biological Origin: E.coli
• Biological Activity: Fully biologically active when compared to standard. Determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 5.0-10.0 ng/ml. Less than 1EU/μg of rHuFractalkine/CX3CL1 as determined by LAL method.
• Target: CX3CL1
• Conjugation: Unconjugated
• MW: 8.5 kDa
• Purity: > 97% pure by SDS-PAGE and HPLC analyses.
• Protein Sequence: QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Fractalkine

SDS-PAGE analysis of Human CX3CL1 protein

Quick Database Links

Gene Symbol

CX3CL1