Human DFFA protein

SKU: orb391618

Description

Recombinant human DFFA protein is produced by our E. coli expression system and the target gene encoding Met1-Thr331 is expressed with a 6His tag at the N-terminus.

Research Area

Product Categories/Products/Proteins,Product Categories/Research Area,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Product Categories/Proteins,Cancer

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: DFFA
• Reactivity: Human
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MGSSHHHHHH SSGLVPRGSH MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid in a 0.2 μM filtered solution of PBS, pH 7.4.
• Buffer/Preservatives: Liquid in a 0.2 μM filtered solution of PBS, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DFF1; DFF45; DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD

Quick Database Links

Gene Symbol

DFFA

UniProt

O00273

NCBI Gene ID

1676