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Description
Research Area
Images & Validation
−
| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Target | ECE1 |
| Reactivity | Human |
| Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | HHHHHHHHQY QTRSPSVCLS EACVSVTSSI LSSMDPTVDP CHDFFSYACG GWIKANPVPD GHSRWGTFSN LWEHNQAIIK HLLENSTASV SEAERKAQVY YRACMNETRI EELRAKPLME LIERLGGWNI TGPWAKDNFQ DTLQVVTAHY RTSPFFSVYV SADSKNSNSN VIQVDQSGLG LPSRDYYLNK TENEKVLTGY LNYMVQLGKL LGGGDEEAIR PQMQQILDFE TALANITIPQ EKRRDEELIY HKVTAAELQT LAPAINWLPF LNTIFYPVEI NESEPIVVYD KEYLEQISTL INTTDRCLLN NYMIWNLVRK TSSFLDQRFQ DADEKFMEVM YGTKKTCLPR WKFCVSDTEN NLGFALGPMF VKATFAEDSK SIATEIILEI KKAFEESLST LKWMDEETRK SAKEKADAIY NMIGYPNFIM DPKELDKVFN DYTAVPDLYF ENAMRFFNFS WRVTADQLRK APNRDQWSMT PPMVNAYYSP TKNEIVFPAG ILQAPFYTRS SPKALNFGGI GVVVGHELTH AFDDQGREYD KDGNLRPWWK NSSVEAFKRQ TECMVEQYSN YSVNGEPVNG RHTLGENIAD NGGLKAAYRA YQNWVKKNGA EHSLPTLGLT NNQLFFLGFA QVWCSVRTPE SSHEGLITDP HSPSRFRVIG SLSNSKEFSE HFRCPPGSPM NPPHKCEVW |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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31.25-2000 pg/mL
11.4 pg/mL
96 T, 48 TECE1 purified MaxPab mouse polyclonal antibody (B01P) [orb2293389]
WB
Human
Mouse
Polyclonal
Unconjugated
50 μgHuman ECE1 Protein, His Tag [orb2701187]
> 95%, determined by SDS-PAGE
This protein contains human ECE1 isoform B (P42892-1) (Gln 90-Trp 770) was expressed with a polyhistidine tag at the N-terminus and expressed from HEK293 Cells.
20 μg, 5 μgECE1 Protein, Human, Recombinant (His) [orb1957725]
98.00%
80 kDa (predicted); 125 kDa (reducing condition, due to glycosylation)
50 μg

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Protocol Information
Human ECE1 protein (orb706925)
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