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Human EGF Protein, His Tag

SKU: orb762429
ActiveBiologically Active

Description

Human EGF Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).

Research Area

Immunology & Inflammation

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
Biological OriginHuman
Biological ActivityImmobilized Human EGF Protein, His Tag, premium grade at 2 µg/mL (100 µL/well) can bind Human EGF R, Fc Tag with a linear range of 0.2-5 ng/mL (QC tested).
TargetEGF
ReactivityHuman
TagN-10×His
Expression RegionAsn 971 - Arg 1023
Endotoxins0.1 EU per μg
ConjugationUnconjugated
MW8.3 kDa
Purity95%
Protein SequenceNP_001954.2

Storage & Handling

Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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Quality Guarantee

Quality Guarantee

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide

Available Sizes

Select a size below

100 μg
$ 260.00
1 mg
$ 420.00
DispatchUsually dispatched within 5-10 working days
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