Human Endosialin(CD248) ELISA Kit

SKU: orb2937733

Description

Human Endosialin(CD248) ELISA Kit

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD248. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD248. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD248, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD248 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.312-20 ng/mL
• Sensitivity: 0.079 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

Endosialin;CD248;Tumor endothelial marker 1;CD164L1, TEM1

Quick Database Links

UniProt

Q9HCU0