Human Eotaxin protein

SKU: orb80123

Description

Eotaxin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8345.9 Dalton. The CCL11 is purified by proprietary chromatographic techniques.

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.013 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of Eotaxin as Reference Standard.

Key Properties

• Biological Origin: E.coli
• Biological Activity: The activity is determined by the chemoattract of human PBE (peripheral blood eosinophils) at concentration between 0.1-10 ng/ml.
• Target: Eotaxin
• Conjugation: Unconjugated
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Ala-Ser-Val.

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The CCL11 was lyophilized from concentrated (1mg/ml) solution in water containing no additives.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Small inducible cytokine A11 protein, CCL11 protein, Eosinophil chemotactic protein protein, chemokine (C-C motif) ligand 11 protein, SCYA11 protein, MGC22554 protein

SDS-PAGE analysis of Human Eotaxin protein

Quick Database Links

Gene Symbol

Eotaxin