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Human EphA4 Protein, His Tag

SKU: orb612090

Description

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EF1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Engaged by the ligand ephrin-A5/EF5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EF2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

Research Area

Immunology & Inflammation

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.3 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
Biological OriginHuman
TargetEphA4
ReactivityHuman
TagC-10×His
Expression RegionVal 20 - Thr 547
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW60.3 kDa
Purity95%
Protein SequenceNP_004429.1

Storage & Handling

Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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Available Sizes

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100 μg
$ 660.00
1 mg
$ 3,860.00
DispatchUsually dispatched within 5-10 working days
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