Human GDF-15 Protein, His Tag

SKU: orb624215

Description

Human GDF-15, His Tag is expressed from E. coli cells. It contains AA Ala 197 - Ile 308 (Accession # Q99988-1).

Research Area

Disease Biomarkers

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.3 kDa. The protein migrates as 15 kDa under reducing (R) condition, and 27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE)

Key Properties

• Expression System: E. coli
• Biological Origin: Human
• Biological Activity: Immobilized Human GDF-15, His Tag at 1 µg/mL (100 µL/well) can bind Human GFR alpha-like, Fc Tag with a linear range of 0.4-25 ng/mL (QC tested).
• Target: GDF-15
• Reactivity: Human
• Tag: N-10×His
• Expression Region: Ala 197 - Ile 308
• Endotoxins: 0.2 EU per μg
• Conjugation: Unconjugated
• MW: 14.3 kDa
• Purity: 90%
• Protein Sequence: NP_004855.2

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: 30% ACN, 0.085% TFA
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Human GDF-15, His Tag (Cat. No. orb624215) at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag (Cat. No. orb1146970) with a linear range of 0.4-25 ng/mL (QC tested).

Quick Database Links

Gene Symbol

GDF-15

UniProt

Q99988

RefSeq (Protein):NP_004855.2