Human Granzyme B (GZMB) ELISA Kit

SKU: orb775522

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme B(GZMB).

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GZMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GZMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GZMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GZMB in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 2.7 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

GZM-B ELISA Kit, HLP ELISA Kit, CTLA1 ELISA Kit, CCPI ELISA Kit, CGL1 ELISA Kit, CSP-B ELISA Kit, CSPB ELISA Kit, CTSGL1 ELISA Kit, SECT ELISA Kit, Granzyme 2 ELISA Kit, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1 ELISA Kit, Fragmentin 2 ELISA Kit, Cytotoxic Serine Protease B ELISA Kit

Quick Database Links

UniProt

P10144