Human HGF Protein, His Tag

SKU: orb700640

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Description

Human HGF, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes

This protein carries a polyhistidine tag at the C-terminus. The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Biological Origin	Human
Biological Activity	Human HGF R, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF, His Tag, premium grade with an affinity constant of 0.486 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Target	HGF
Reactivity	Human
Tag	C-10×His
Expression Region	Gln 32 - Ser 728
Endotoxins	0.1 EU per μg
Conjugation	Unconjugated
MW	81.6 kDa (53.7 kDa\|27.9 kDa)
Purity	90%
Protein Sequence	Gln 32 - Ser 728

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Human HGFAC Protein, His Tag [orb2701646]

Purity:	> 95%, determined by SDS-PAGE
Source:	This protein contains the human HGF activator precursor (NP_0015191) (Met 1-Ser 655) was expressed with a fused polyhistidine-tag at the C-terminus and expressed from HEK293 Cells.

Sizes Available: 10 μg, 20 μg

Recombinant Human HGF R/c-MET Protein, His Tag [orb1551050]

Purity:	> 95% by SDS-PAGE.
Source:	Recombinant Human HGF R/c-MET Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Glu25-Thr932) of human HGF R/c-MET (Accession #NP_000236.2) fused with a 6��His Tag at the C-terminus.

Sizes Available: 100 μg, 50 μg

Recombinant Human HGF/Hepatocyte Growth Factor Protein, His Tag [orb1550840]

Purity:	> 95% by SDS-PAGE.
Source:	Recombinant Human HGF/Hepatocyte Growth Factor Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Gln32-Ser728) of human HGF/Hepatocyte Growth Factor (Accession #P14210) fused with a 6��His Tag at the C-terminus.

Sizes Available: 50 μg

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Human HGF R, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF, His Tag, premium grade (Cat. No. orb700640) with an affinity constant of 0.486 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Quick Database Links

Gene Symbol

HGF

UniProt

P14210

UniProt Details

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No UniProt data available

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