Human IGF-I R Protein, His Tag

SKU: orb511230

Description

Human IGF-I R, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).

Research Area

Disease Biomarkers

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.0 kDa and 23.8 kDa respectively. The protein migrates as 35-45 kDa (partial β chain), 110-120 kDa (α chain) and 130 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

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Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Human IGF-I R, His Tag immobilized on CM5 Chip can bind Biotinylated Human IGF-I, His, with an affinity constant of 68 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Target: IGF-I R
• Reactivity: Human
• Tag: C-10×His
• Expression Region: Glu 31 - Asn 932
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 104.8 kDa
• Purity: 90%
• Protein Sequence: NP_000866.1

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

IGF-I R

UniProt

P08069

RefSeq (Protein):NP_000866.1