Human IGFBP-3 Protein, His Tag

SKU: orb1146966

Description

Human IGFBP-3, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 28 - Lys 291 (Accession # P17936-1).

Research Area

Disease Biomarkers

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.6 kDa. The protein migrates as 40-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Biotinylated Human IGF-II, Fc Tag at 2 µg/mL (100 µL/well) on streptavidin precoated (1 µg/well) plate can bind Human IGFBP-3, His Tag with a linear range of 0.01-0.313 µg/mL (QC tested).
• Target: IGFBP-3
• Reactivity: Human
• Tag: C-10×His
• Expression Region: Gly 28 - Lys 291
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 30.6 kDa
• Purity: 90%
• Protein Sequence: NP_000589.2

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Biotinylated Human IGF-II, Avitag, Fc Tag at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. orb668907) precoated (1 μg/well) plate can bind Human IGFBP-3, His Tag (Cat. No. orb1146966) with a linear range of 0.01-0.313 μg/mL (QC tested).

Quick Database Links

Gene Symbol

IGFBP-3

UniProt

P17936

RefSeq (Protein):NP_000589.2