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Human IGFBP-3 Protein, His Tag

SKU: orb1146966
ActiveBiologically Active

Description

Human IGFBP-3, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 28 - Lys 291 (Accession # P17936-1).

Research Area

Disease Biomarkers

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.6 kDa. The protein migrates as 40-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
Biological OriginHuman
Biological ActivityImmobilized Biotinylated Human IGF-II, Fc Tag at 2 µg/mL (100 µL/well) on streptavidin precoated (1 µg/well) plate can bind Human IGFBP-3, His Tag with a linear range of 0.01-0.313 µg/mL (QC tested).
TargetIGFBP-3
ReactivityHuman
TagC-10×His
Expression RegionGly 28 - Lys 291
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW30.6 kDa
Purity90%
Protein SequenceNP_000589.2

Storage & Handling

Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide

Available Sizes

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100 μg
$ 750.00
1 mg
$ 2,970.00
DispatchUsually dispatched within 5-10 working days
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