Human IGFBP-4 Protein, His Tag

SKU: orb348805

Description

Human IGFBP-4, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Glu 258 (Accession # P22692-1).

Research Area

Disease Biomarkers

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.9 kDa. The protein migrates as 33-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human IGFBP-4, His Tag at 2 µg/mL (100 µL/well) can bind Human IGF-I, Fc Tag with a linear range of 1-16 ng/mL (QC tested).
• Target: IGFBP-4
• Reactivity: Human
• Tag: C-10×His
• Expression Region: Asp 22 - Glu 258
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 27.9 kDa
• Purity: 95%
• Protein Sequence: NP_001543.2

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of Human IGFBP-4 protein

Immobilized Human IGFBP-4, His Tag (Cat. No. orb348805) at 2 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (Cat. No. orb257564) with a linear range of 1-16 ng/mL (QC tested).

Quick Database Links

Gene Symbol

IGFBP-4

UniProt

P22692

RefSeq (Protein):NP_001543.2