Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

SKU: orb347240

Description

Human IgG (H&L) antibody (Alkaline Phosphatase)

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Reactivity: Human
• Application Notes:
  Anti-Human IgG (H&L) Alkaline Phosphatase has been tested by dot blot and ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,500 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins..
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

goat anti-Human IgG Alkaline Phosphatase Conjugation, goat anti-Human IgG ALP conjugate, goat anti-human IgG Alk Phos conjugated antibody

Dot Blot of Goat anti-Human IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG Antibody Alkaline Phosphatase Conjugated Pre-Absorbed at 1:1000 for 1 HR at RT. Block: orb348637 for 1 HR at RT.