Human IL-1 alpha Protein, His Tag

SKU: orb867311

Description

Human IL-1 alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 113 - Ala 271 (Accession # P01583-1).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.9 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human IL-1 RI Protein, Fc Tag at 10 µg/mL (100 µL/well) can bind Human IL-1 alpha, His Tag with a linear range of 0.3-2.5 µg/mL (QC tested).
• Target: IL-1 alpha
• Reactivity: Human
• Tag: C-10×His
• Expression Region: Ser 113 - Ala 271
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 19.9 kDa
• Purity: 95%
• Protein Sequence: NP_000566.3

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Human IL-1 RI Protein, Fc Tag at 10 μg/mL (100 μL/well) can bind Human IL-1 alpha, His Tag (Cat. No. orb867311) with a linear range of 0.3-2.5 μg/mL (QC tested).

Quick Database Links

Gene Symbol

IL-1 alpha

UniProt

P01583

RefSeq (Protein):NP_000566.3