Human IL-10 Protein, His Tag

SKU: orb334903

Description

Human IL-10, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # NP_000563.1).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.5 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).

Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human IL-10, His Tag at 5 µg/mL (100 µL/well) can bind Biotinylated Human IL-10 R alpha, Fc, with a linear range of 0.2-10 ng/mL (QC tested).
• Target: IL-10
• Reactivity: Human
• Tag: N-6×His
• Expression Region: Ser 19 - Asn 178
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 19.5 kDa
• Purity: 95%
• Protein Sequence: NP_000563.1

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of Human IL-10 protein

Immobilized Human IL-10, His Tag (Cat. No. orb334903) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc, Avitag with a linear range of 0.2-10 ng/mL (QC tested).

Quick Database Links

Gene Symbol

IL-10

UniProt

P22301

RefSeq (Protein):NP_000563.1