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Description
Research Area
Immunology & Inflammation
Images & Validation
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Item 1 of 2
| Application Notes |
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Key Properties
−| Expression System | HEK293 |
|---|---|
| Biological Origin | Human |
| Biological Activity | Immobilized Human IL-10, His Tag at 5 µg/mL (100 µL/well) can bind Biotinylated Human IL-10 R alpha, Fc, with a linear range of 0.2-10 ng/mL (QC tested). |
| Target | IL-10 |
| Reactivity | Human |
| Tag | N-6×His |
| Expression Region | Ser 19 - Asn 178 |
| Endotoxins | 1.0 EU per μg |
| Conjugation | Unconjugated |
| MW | 19.5 kDa |
| Purity | 95% |
| Protein Sequence | NP_000563.1 |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Human IL10 Protein, His Tag [orb1471796]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 19.5 kDa after removal of the signal peptide. The apparent molecular mass of His-IL10 is approximately 15-25 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000563.1 |
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
Available Sizes
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