Human IL-12 R beta 1 Protein, Fc Tag

SKU: orb570282

Description

Human IL-12 R beta 1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 540 (Accession # P42701-1).

Research Area

Disease Biomarkers

Images & Validation

• Application Notes:
  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 83.5 kDa. The protein migrates as 95-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Human IL-12 R beta 1, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B & IL-12A Heterodimer Protein, His Tag & Flag Tag with an affinity constant of 2.06 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Target: IL-12 R beta 1
• Reactivity: Human
• Tag: C-Fc
• Expression Region: Cys 24 - Glu 540
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 83.5 kDa
• Purity: 90%
• Protein Sequence: NP_005526.1

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Human IL-12 R beta 1, Fc Tag (Cat. No. orb570282) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B & IL-12A Heterodimer Protein, His Tag & Flag Tag with an affinity constant of 2.06 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Quick Database Links

Gene Symbol

IL-12 R beta 1

UniProt

P42701

RefSeq (Protein):NP_005526.1