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Human IL-12B Protein, Fc Tag

SKU: orb257708
ActiveBiologically Active

Description

Human IL-12B, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # AAH67499).

Research Area

Immunology & Inflammation

Images & Validation

Application Notes
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 61.3 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
Biological OriginHuman
Biological ActivityImmobilized Human IL-12 R beta 1, His Tag at 2 µg/mL (100 µL/well) can bind Human IL-12B, Fc Tag with a linear range of 0.039-0.625 µg/mL (QC tested).
TargetIL-12B
ReactivityHuman
TagC-Fc
Expression RegionIle 23 - Ser 328
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW61.3 kDa
Purity95%
Protein SequenceAAH67499

Storage & Handling

Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesTris with Glycine, Arginine and NaCl, pH 7.5
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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Human IL-12B Protein, Fc Tag

SDS-PAGE analysis of Human IL-12B protein

Human IL-12B Protein, Fc Tag

Immobilized Human IL-12 R beta 1, His Tag (Cat. No. orb570283) at 2 μg/mL (100 μL/well) can bind Human IL-12B, Fc Tag (Cat. No. orb257708) with a linear range of 0.039-0.625 μg/mL (QC tested).

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqAAH67499

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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Human IL-12B Protein, Fc Tag (orb257708)

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20 μg
$ 420.00
50 μg
$ 770.00
1 mg
$ 4,830.00
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