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Human IL-12B Protein, Fc Tag

SKU: orb257708
ActiveBiologically Active

Description

Human IL-12B, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # AAH67499).

Research Area

Immunology & Inflammation

Images & Validation

Application Notes
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 61.3 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
Biological OriginHuman
Biological ActivityImmobilized Human IL-12 R beta 1, His Tag at 2 µg/mL (100 µL/well) can bind Human IL-12B, Fc Tag with a linear range of 0.039-0.625 µg/mL (QC tested).
TargetIL-12B
ReactivityHuman
TagC-Fc
Expression RegionIle 23 - Ser 328
Endotoxins1.0 EU per μg
ConjugationUnconjugated
MW61.3 kDa
Purity95%
Protein SequenceAAH67499

Storage & Handling

Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesTris with Glycine, Arginine and NaCl, pH 7.5
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqAAH67499

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide

Available Sizes

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20 μg
$ 420.00
50 μg
$ 770.00
1 mg
$ 4,830.00
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