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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Biological Origin | Human |
| Biological Activity | Loaded Human IL-12 R beta 1, Fc Tag on Protein A Biosensor, can bind Human IL-12B, His Tag with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Target | IL-12B |
| Reactivity | Human |
| Tag | C-10×His |
| Expression Region | Ile 23 - Ser 328 |
| Endotoxins | 1.0 EU per μg |
| Conjugation | Unconjugated |
| MW | 36.6 kDa |
| Purity | 95% |
| Protein Sequence | AAH67499.1 |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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SDS-PAGE analysis of Human IL-12B protein
Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. orb570282) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. orb257709) with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | AAH67499.1 |
|---|
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Protein Handling and Storage Guide
Protein Handling Guide
Human IL-12B Protein, His Tag (orb257709)
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