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Description
Images & Validation
−| Tested Applications | FA, HPLC, SDS-PAGE |
|---|---|
| Application Notes |
Key Properties
−| Biological Origin | E.coli |
|---|---|
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of mouse MC-9 cells was found to be 0.5 ng/ml, corresponding to Specific Activity of 3,000,000 IU/mg. |
| Target | IL10 |
| Conjugation | Unconjugated |
| Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr. |
Storage & Handling
−| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.. The protein was lyophilized from concentrated (1mg/ml) solution containing no additives. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IL10 protein (Active) [orb358975]
> 95% as determined by SDS-PAGE and HPLC.
18.6 kDa
E.Coli
500 μg, 10 μg, 100 μgHuman IL10 Protein, His Tag [orb1471796]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 19.5 kDa after removal of the signal peptide. The apparent molecular mass of His-IL10 is approximately 15-25 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μgHuman IL10 Protein, hFc Tag [orb1291070]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-IL10 is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgHuman IL10 protein [orb391908]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μg

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SDS-PAGE analysis of Human IL10 protein
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Human IL10 protein (orb80067)
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