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Human IL10 protein

SKU: orb80067

Description

IL10 Human Recombinant produced in E.coli is non covalent dimer of polypeptide chains containing 161 amino acids each and having a molecular mass of 18606.29 Dalton.The IL-10 is purified by proprietary chromatographic techniques.

Images & Validation

Tested ApplicationsFA, HPLC, SDS-PAGE
Application Notes
< strong>IHC: E.coli

Key Properties

Biological OriginE.coli
Biological ActivityThe ED50 as determined by the dose-dependant stimulation of mouse MC-9 cells was found to be 0.5 ng/ml, corresponding to Specific Activity of 3,000,000 IU/mg.
TargetIL10
ConjugationUnconjugated
Purity> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
Protein SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.

Storage & Handling

StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/PreservativesThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.. The protein was lyophilized from concentrated (1mg/ml) solution containing no additives.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

B-TCGF protein, CSIF protein, TGIF protein, IL-10 protein, IL10A protein, MGC126450 protein, MGC126451 protein, Cytokine synthesis inhibitory factor protein

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Human IL10 protein

SDS-PAGE analysis of Human IL10 protein

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol
HPLC
High-Performance Liquid Chromatography
View Protocol

Human IL10 protein (orb80067)

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10 μg
$ 340.00
100 μg
$ 1,480.00
500 μg
$ 3,300.00
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