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Description
Images & Validation
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Key Properties
−| Source | This protein contains the p35 subunit of human IL12, termed as IL12A (P29459) (Met 1-Ser 219) was fused with the Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells. |
|---|---|
| Expression System | HEK293 Cells |
| Target | IL12A |
| Reactivity | Human |
| Endotoxins | > 95%, determined by SDS-PAGE |
| Purity | > 95%, determined by SDS-PAGE |
Storage & Handling
−| Expiration Date | 6 months from date of receipt. |
|---|---|
| Disclaimer | For research use only |
Alternative Names
−IL12A, interleukin 12A, P35, CLMF, NFSK, NKSF1, IL-12A
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Human IL12A and IL12B Heterodimer Protein, hFc Tag and His Tag [orb1743293]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide.
Mammalian
50 μg, 10 μg, 100 μg Human IL12A & IL27B Protein, His & Fc Tag [orb2698055]
> (59.8+33.3)%, determined by SDS-PAGE
This protein contains the p35 subunit of human IL12, termed as IL12A (NP_0008732) (Met1-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; This protein contains the human IL27B, (NP_0057462) (Met1-Lys229) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2 . The two plasmids were co-expressed and the heterodimer was purified and expressed from HEK293 Cells.
100 μg, 20 μgHuman IL12A & IL12B Protein, Fc Tag [orb2698078]
> 95%, determined by SDS-PAGE
This protein contains the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; This protein contains the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
20 μg, 5 μgRecombinant Human IL12A/NKSF1 Protein, Fc & His Tag [orb1551291]
> 95% by SDS-PAGE.
Recombinant Human IL12A/NKSF1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg23-Ser219) of human IL12A/NKSF1 (Accession #NP_002178.2) fused with an Fc, 6��His Tag at the C-terminus.
50 μg, 100 μg
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Quick Database Links
Gene Symbol
IL12A
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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