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Human IL1RA protein

SKU: orb80054

Description

Recombinant human IL1RA protein

Images & Validation

Tested ApplicationsFA, HPLC, SDS-PAGE
Application Notes
< strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-1 ra as Reference Standard.

Key Properties

Biological OriginE.coli
Biological ActivityThe ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml.
TargetIL1RA
ConjugationUnconjugated
Purity> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
Protein SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly.

Storage & Handling

StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/PreservativesLyophilized protein with no additives.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

DIRA protein, ICIL 1RA protein, ICIL-1RA protein, ICIL1RA protein, IL-1ra protein, IL-1ra3 protein, IL-1RN protein, IL1 inhibitor protein, IL1F3 protein, IL1RA protein, IL1RA_HUMAN protein, IL1RN (IL1F3) protein, IL1 RN protein, IL1RN protein, IL1 RA protein, Interleukin 1 receptor antagonist protein, Interleukin-1 receptor antagonist protein protein, Intracellular IL 1 receptor antagonist type II protein, Intracellular interleukin 1 receptor antagonist (icIL 1ra) protein, IRAP protein, MGC10430 protein, MVCD4 protein, Type II interleukin 1 receptor antagonist protein

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Human IL1RA protein

SDS-PAGE analysis of Human IL1RA protein

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol
HPLC
High-Performance Liquid Chromatography
View Protocol

Human IL1RA protein (orb80054)

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Available Sizes

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100 μg
$ 400.00
500 μg
$ 800.00
DispatchUsually dispatched within 2-3 weeks
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