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Human IL25 protein

SKU: orb391967

Description

Recombinant human IL25 protein is produced by our mammalian expression system and the target gene encoding Tyr33-Gly177 is expressed with a 6His tag at the C-terminus.

Research Area

Product Categories/Products/Proteins,Product Categories/Research Area,Product Categories/Proteins,Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
TargetIL25
ReactivityHuman
EndotoxinsEndotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceYSHWPSCCPS KGQDTSEELL RWSTVPVPPL EPARPNRHPE SCRASEDGPL NSRAISPWRY ELDRDLNRLP QDLYHARCLC PHCVSLQTGS HMDPRGNSEL LYHNQTVFYR RPCHGEKGTH KGYCLERRLY RVSLACVCVR PRVMGVDHHH HHH

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 1mM EDTA, pH 8.0.
Buffer/PreservativesLyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 1mM EDTA, pH 8.0.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

IL17E; Interleukin-25; IL-25; Interleukin-17E; IL-17E

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 270.00
50 μg
$ 650.00
500 μg
$ 2,970.00
DispatchUsually dispatched within 5-10 working days
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