Human IL29 protein

SKU: orb82616

Description

IL28A, IL28B, and IL29, also named interferon lambda2 (IFN lambda 2), IFN lambda 3, and IFN lambda 1, respectively, are newly identified class II cytokine receptor ligands that are distantly related to members of the IL10 family (11-13% aa sequence identity) and the type I IFN family (15-19% aa sequence identity). The expression of IL28A, B, and IL29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL10 R beta) and a novel IL28 receptor alpha (IL28 Ralpha, also known as IFN lambdaR1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation.

Images & Validation

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Tested Applications	FA, HPLC, SDS-PAGE
Application Notes	Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Key Properties

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Biological Origin	E.coli
Biological Activity	Fully biologically active when compared to standard. The ED50 is determined in an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is typically 1-5 ng/ml.
Target	IL29
Conjugation	Unconjugated
MW	19.8 kDa
Purity	> 97% pure by SDS-PAGE and HPLC analyses.
Protein Sequence	PTSKPTTTGK GCHIGRFKSL SPQELASFKK ARDALEESLK LKNWSCSSPV FPGNWDLRLL QVRERPVALE AELALTLKVL EAAAGPALED VLDQPLHTLH HILSQLQACI QPQPTAGPRP RGRLHHWLHR LQEAPKKESA GCLEASVTFN LFRLLTRDLK YVADGNLCLR TSTHPEST

Storage & Handling

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Storage	Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 130mM NaCl. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IFN lambda1