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Human LEP protein

SKU: orb392069

Description

Recombinant human LEP protein is produced by our E. coli expression system and the target gene encoding Val22-Cys167 is expressed.

Research Area

Product Categories/Products/Proteins,Product Categories/Research Area,Product Categories/Proteins,Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetLEP
ReactivityHuman
EndotoxinsEndotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Protein SequenceVPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Buffer/PreservativesLyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

OB; OBS; Leptin; Obese protein; Obesity factor

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Datasheet
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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human LEP protein (orb392069)

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Available Sizes

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10 μg
$ 100.00
50 μg
$ 240.00
500 μg
$ 740.00
DispatchUsually dispatched within 5-10 working days
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