Human M-CSF R Protein, His Tag

SKU: orb257371

Description

Human M-CSF R, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # NP_005202.2).

Research Area

Disease Biomarkers

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Biotinylated Human M-CSF, His, immobilized on SA Chip can bind Human M-CSF R, His Tag with an affinity constant of 0.309 µM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Target: M-CSF R
• Reactivity: Human
• Tag: C-6×His
• Expression Region: Ile 20 - Glu 512
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 55.3 kDa
• Purity: 96%
• Protein Sequence: NP_005202.2

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of Human M-CSF R protein

Biotinylated Human M-CSF, His, Avitag immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. orb257371) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Quick Database Links

Gene Symbol

M-CSF R

UniProt

P07333

RefSeq (Protein):NP_005202.2