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Description
Research Area
Product Categories/Products/Proteins,Product Categories/Research Area,Product Categories/Proteins,Epigenetics
Images & Validation
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| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | E. coli |
|---|---|
| Target | MB |
| Reactivity | Human |
| Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | MGLSDGEWQL VLNVWGKVEA DIPGHGQEVL IRLFKGHPET LEKFDKFKHL KSEDEMKASE DLKKHGATVL TALGGILKKK GHHEAEIKPL AQSHATKHKI PVKYLEFISE CIIQVLQSKH PGDFGADAQG AMNKALELFR KDMASNYKEL GFQGHHHHHH |
Storage & Handling
−| Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, 1mM DTT, 20% glycerol, pH 8.0. |
| Buffer/Preservatives | Liquid in a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, 1mM DTT, 20% glycerol, pH 8.0. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Myoglobin protein
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 29.8 kDa after removal of the signal peptide. The apparent molecular mass of KI67(1206-1238)-hFc is approximately 35-55 kDa due to glycosylation.
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
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