Human MCP2 protein

SKU: orb80141

Description

Monocyte Chemotactic Protein-2 Human Recombinant produced in E.coli is non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton. The MCP2 is purified by proprietary chromatographic techniques.

Images & Validation

• Tested Applications: FA, HPLC, SDS-PAGE

Key Properties

• Biological Origin: E.coli
• Biological Activity: The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with 500ng/mL of recombinant human MCP-2. Human MCP-2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100 ng/mL.
• Target: MCP 2
• Conjugation: Unconjugated
• Purity: > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The protein was lyophilized from concentrated (1mg/ml) sterile solution containing no additives.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Small inducible cytokine A8 protein, CCL8 protein, Monocyte chemotactic protein 2 protein, MCP-2 protein, Monocyte chemoattractant protein 2 protein, HC14 protein, chemokine (C-C motif) ligand 8 protein, MCP2 protein, SCYA8 protein, SCYA10 protein

SDS-PAGE analysis of Human MCP2 protein

Quick Database Links

Gene Symbol

MCP 2