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Description
Research Area
Product Categories/Products/Proteins,Product Categories/Research Area,Epigenetics,Product Categories/Proteins,Developmental Biology
Images & Validation
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| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Target | SEMA4D |
| Reactivity | Human |
| Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | MAFAPIPRIT WEHREVHLVQ FHEPDIYNYS ALLLSEDKDT LYIGAREAVF AVNALNISEK QHEVYWKVSE DKKAKCAEKG KSKQTECLNY IRVLQPLSAT SLYVCGTNAF QPACDHLNLT SFKFLGKNED GKGRCPFDPA HSYTSVMVDG ELYSGTSYNF LGSEPIISRN SSHSPLRTEY AIPWLNEPSF VFADVIRKSP DSPDGEDDRV YFFFTEVSVE YEFVFRVLIP RIARVCKGDQ GGLRTLQKKW TSFLKARLIC SRPDSGLVFN VLRDVFVLRS PGLKVPVFYA LFTPQLNNVG LSAVCAYNLS TAEEVFSHGK YMQSTTVEQS HTKWVRYNGP VPKPRPGACI DSEARAANYT SSLNLPDKTL QFVKDHPLMD DSVTPIDNRP RLIKKDVNYT QIVVDRTQAL DGTVYDVMFV STDRGALHKA ISLEHAVHII EETQLFQDFE PVQTLLLSSK KGNRFVYAGS NSGVVQAPLA FCGKHGTCED CVLARDPYCA WSPPTATCVA LHQTESPSRG LIQEMSGDAS VCPDKSKGSY RQHFFKHGGT AELKCSQKSN LARVFWKFQN DVLKAESPKY GLMGRKNLLI FNLSEGDSGV YQCLSEERVK NKTVFQVVAK HVLEVKVVPK PVVAPTLSVV QTEGSRIATK VLVASTQGSS PPTPAVQATS SGAITLPPKP APTGTSCEPK IVINTVPQLH SEKTMYLKSS DNRVDHHHHH H |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−C9orf164; CD100; SEMAJ; Semaphorin-4D; A8; BB18; GR3; CD100
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Human SEMA4D Protein, His Tag [orb1290824]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 80 kDa after removal of the signal peptide. The apparent molecular mass of SEMA4D-His is approximately 95-130 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 1
Human SEMA4D protein [orb392440]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μg
Human CD100 (HEK) Protein [orb424546]
Greater than 95% as determined by SDS-PAGE.
HEK293 cells
1 mg, 5 μg, 20 μg
Quality Guarantee
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
Available Sizes
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