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Description
Research Area
Product Categories/Products/Proteins,Product Categories/Research Area,Product Categories/Proteins,Epigenetics
Images & Validation
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| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Target | SIRPA |
| Reactivity | Human |
| Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | EEELQVIQPD KSVSVAAGES AILHCTVTSL IPVGPIQWFR GAGPARELIY NQKEGHFPRV TTVSESTKRE NMDFSISISN ITPADAGTYY CVKFRKGSPD TEFKSGAGTE LSVRAKPSAP VVSGPAARAT PQHTVSFTCE SHGFSPRDIT LKWFKNGNEL SDFQTNVDPV GESVSYSIHS TAKVVLTRED VHSQVICEVA HVTLQGDPLR GTANLSETIR VPPTLEVTQQ PVRAENQVNV TCQVRKFYPQ RLQLTWLENG NVSRTETAST VTENKDGTYN WMSWLLVNVS AHRDDVKLTC QVEHDGQPAV SKSHDLKVSA HPKEQGSNTA AENTGSNERD IEGRMDEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BIT protein, MFR protein, MYD1 protein, PTPNS1 protein, SHPS1 protein, SIRP protein, Tyrosine-protein phosphatase non-receptor type substrate 1 protein, SHP substrate 1 protein, SHPS-1 protein, Brain Ig-like molecule with tyrosine-based activation motifs protein, Bit protein, CD172 antigen-like family member A protein, Inhibitory receptor SHPS-1 protein, Macrophage fusion receptor protein, MyD-1 antigen protein, Signal-regulatory protein alpha-1 protein, Sirp-alpha-1 protein, Signal-regulatory protein alpha-2 protein, Sirp-alpha-2 protein, Signal-regulatory protein alpha-3 protein, Sirp-alpha-3 protein, p84 protein, CD172a protein
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 70-98 kDa after removal of the signal peptide.
Mammalian
10 μg, 50 μg, 100 μg

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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis









