Human Sonic Hedgehog Protein, His Tag

SKU: orb257838

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Description

Human Sonic Hedgehog, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184).

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Biological Origin	Human
Target	Sonic Hedgehog
Reactivity	Human
Tag	C-6×His
Expression Region	Cys 24 - Gly 197
Endotoxins	0.2 EU per μg
Conjugation	Unconjugated
MW	20.4 kDa
Purity	90%
Protein Sequence	NP_000184

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Human SHH Protein, His Tag [orb2701586]

Purity:	> 97%, determined by SDS-PAGE
Source:	This protein contains the amino acid sequence (Cys 198-Ser 462) of human SHH (Q15465), that is Sonic hedgehog protein C-product, was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus and expressed from HEK293 Cells.

Sizes Available: 20 μg, 5 μg

Human SHH Protein, His Tag [orb2701587]

Purity:	> 95%, determined by SDS-PAGE
Source:	This protein contains the amino acid sequence (Met 1-Gly 197) of human SHH (Q15465), that is Sonic hedgehog protein N-product, was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells.

Sizes Available: 20 μg, 5 μg

Human FGF-4 Protein, His Tag [orb1733261]
Conjugation:
Unconjugated
Purity:
90%
MW:
19.8 kDa
Sizes Available: 1 mg, 100 μg

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SDS-PAGE analysis of Human Sonic Hedgehog protein

Quick Database Links

Gene Symbol

Sonic Hedgehog

UniProt

Q15465

RefSeq (Protein):NP_000184

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000184

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Protein Handling Guide

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